Abstract: A P(GMA-co-HEMA)/SiO₂ macroporous composite was synthesized by in-situ polymerization followed by solvent evaporation via a large-sized macroporous SiO₂ material as substrate and glycidyl methacrylate(GMA), 2-hydroxyethyl methacrylate(HEMA) as the functional monomers. Samples were characterized by SEM, EDS, BET, FTIR and TG-DTA. The P(GMA-co-HEMA)/SiO₂ macroporous composite was employed as support for the immobilization of lipase from Candida rugose (CRL). The results show that the strong capillarity effect of the macroporous SiO₂ material makes the copolymer coating on its pore wall uniformly, forming the P(GMA-co-HEMA)/SiO₂ composite nano-film. The loading amount and the hydrophilicity of the copolymer can be adjusted by changing the concentration of the monomers and the volume ratio of the monomers, respectively. When the volume concentration of monomers is 10% and the volume ratio of GMA to HEMA is 9∶1, the immobilized lipase reaches its highest specific activity of 3 886.9 U/g and remains 68.7% of its initial activity after 8 consecutive batches of reaction with the substrate.